http://neurodudes.com/2005/09/07/real-time-feedback-in-meas-review-paper/ at the intersection of neuroscience and AI. Sat, 05 Jul 2008 12:26:07 +0000 http://wordpress.org/?v=2.5.1 http://neurodudes.com/2005/09/07/real-time-feedback-in-meas-review-paper/#comment-848 Neville Wed, 07 Sep 2005 15:26:03 +0000 http://neurodudes.com/2005/09/07/real-time-feedback-in-meas-review-paper/#comment-848 Very interesting stuff. I haven't read through most of the paper but I highly recommend the culture sections to those involved in neuron culture. The dicussion is very frank and readable (haven't seen too many papers that have sections called "Why Neuron Cultures Die"... more of them should though!) Lots of good info on humidity and osmolarity issues, including an innovative technique for sealing the culture dishes with a Teflon-like polymer for long-term studies. Also, I never knew that the concentration of oxygen is so low in the brain (1-5%); Potter et al. claim that they get nice results when they incubate in more physiological O2 concentrations (instead of just staying with the atmospheric 20%). There are also some nice tips on building a microscope incubator (they recommend this company for insulating material: <a href="http://reflectixinc.com/" rel="nofollow">Reflectix Inc</a>), something I have also struggled with, and on combining 2-photon imaging with long-term culture. Very interesting stuff. I haven’t read through most of the paper but I highly recommend the culture sections to those involved in neuron culture. The dicussion is very frank and readable (haven’t seen too many papers that have sections called “Why Neuron Cultures Die”… more of them should though!) Lots of good info on humidity and osmolarity issues, including an innovative technique for sealing the culture dishes with a Teflon-like polymer for long-term studies. Also, I never knew that the concentration of oxygen is so low in the brain (1-5%); Potter et al. claim that they get nice results when they incubate in more physiological O2 concentrations (instead of just staying with the atmospheric 20%). There are also some nice tips on building a microscope incubator (they recommend this company for insulating material: Reflectix Inc), something I have also struggled with, and on combining 2-photon imaging with long-term culture.

]]>