Comments on: Awesome new voltage-sensitive dye http://neurodudes.com/2005/11/09/awesome-new-voltage-sensitive-dye/ at the intersection of neuroscience and AI. Sat, 30 Aug 2008 04:07:57 +0000 http://wordpress.org/?v=2.6 By: Bayle http://neurodudes.com/2005/11/09/awesome-new-voltage-sensitive-dye/#comment-311475 Bayle Sat, 18 Aug 2007 20:23:47 +0000 http://neurodudes.com/?p=197#comment-311475 > It’s not so hard to get a beautiful signal-to-noise ratio in a culture dish. well actually it is pretty hard :). at least when it comes to VSDs. anyway, even though it's not brain cells, it's a start. now people can start to do it in neurons (or at least they could if a commercial supplier of dipicrylamine was available -- i tried to get my hands on some about a year ago and couldn't find any). i don't know much about it, but I think people do HEK293 first because they're easy to transfect. > It’s not so hard to get a beautiful signal-to-noise ratio in a culture dish.

well actually it is pretty hard :). at least when it comes to VSDs.

anyway, even though it’s not brain cells, it’s a start. now people can start to do it in neurons (or at least they could if a commercial supplier of dipicrylamine was available — i tried to get my hands on some about a year ago and couldn’t find any). i don’t know much about it, but I think people do HEK293 first because they’re easy to transfect.

]]> By: mt http://neurodudes.com/2005/11/09/awesome-new-voltage-sensitive-dye/#comment-311187 mt Sat, 18 Aug 2007 06:16:06 +0000 http://neurodudes.com/?p=197#comment-311187 Last time I checked, HEK293 cells don't carry action potentials and you don't find them in brains. It's not so hard to get a beautiful signal-to-noise ratio in a culture dish. One small step for cell sorting, but not necessarily any big whup for in situ or otherwise in vivo study of brains. Last time I checked, HEK293 cells don’t carry action potentials and you don’t find them in brains. It’s not so hard to get a beautiful signal-to-noise ratio in a culture dish. One small step for cell sorting, but not necessarily any big whup for in situ or otherwise in vivo study of brains.

]]> By: Ted Morse http://neurodudes.com/2005/11/09/awesome-new-voltage-sensitive-dye/#comment-310520 Ted Morse Thu, 16 Aug 2007 19:18:17 +0000 http://neurodudes.com/?p=197#comment-310520 I am interested in developing a microelectron device in which such a voltage sensitive dye might be separated from an aggegrate of cells by a distance of the order of 5-30 nm. Thus, the dye would not be in contact with the cell. Am I correct in assuming that if the dye receives a voltage of the order of 50-100 mV, it will fluoresce? Is this concept of interest? Thanks, Ted Morse Boston Unviersity I am interested in developing a microelectron device in which such a voltage sensitive dye might be separated from an aggegrate of cells by a distance of the order of 5-30 nm. Thus, the dye would not be in contact with the cell. Am I correct in assuming that if the dye receives a voltage of the order of 50-100 mV, it will fluoresce? Is this concept of interest?
Thanks,
Ted Morse
Boston Unviersity

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