Comments on: Optical detection via second harmonic generation

By: bob

bob — Fri, 07 Jul 2006 01:02:16 +0000

S. Baconnier and S.B. Lang, “Calcite microcrystals in the pineal gland of the human brain: second harmonic generators and possible piezoelectric transducers, “ IEEE Transactions on Dielectrics and Electrical Insulation 11, 203-209 (2004).

By: guest

guest — Wed, 05 Apr 2006 21:18:15 +0000

Some people have found luck with SHG and V-dep dyes by adding antioxidants, e.g. in Watt Webb’s group. But I believe they’re mostly focused on Aplysia neurons. Mammalian neurons may need extra help.

By: Neville

Neville — Wed, 05 Apr 2006 17:19:33 +0000

As for the issue with loading in mammalian neurons en masse, I wonder if there’s a way to genetically express a dye with SHG properties. Something similar to the FM dyes?

By: Bayle

Bayle — Wed, 05 Apr 2006 02:24:26 +0000

guest, thanks for the info. in fact i’m working with external dye loading into mammalian neurons right now (for voltage-sensitive imaging, or maybe just calcium), and i was wondering if i should check out this second harmonic stuff. i guess i’ll put it pretty low on the ol’ to-do list.

By: guest

guest — Wed, 05 Apr 2006 00:31:33 +0000

I don’t know anyone who’s gotten it to work with a mammalian neuron, with external dye loading.