Comments on: A genetically encoded fluorescent amino acid http://neurodudes.com/2006/07/02/a-genetically-encoded-fluorescent-amino-acid/ at the intersection of neuroscience and AI. Wed, 07 Dec 2011 00:01:17 +0000 hourly 1 http://wordpress.org/?v=3.2.1 By: Ed http://neurodudes.com/2006/07/02/a-genetically-encoded-fluorescent-amino-acid/comment-page-1/#comment-6376 Ed Fri, 21 Jul 2006 15:12:41 +0000 http://neurodudes.com/2006/07/02/a-genetically-encoded-fluorescent-amino-acid/#comment-6376 Probabilistically, you're likely to stop within a few dozen codons' worth of DNA anyway, given that the two remaining stop codons are 3% of the codons. And the polyadenylation sequence is also likely to halt transcription at the appropriate point, making it so that there is not much more to the mRNA in any case. Probabilistically, you’re likely to stop within a few dozen codons’ worth of DNA anyway, given that the two remaining stop codons are 3% of the codons. And the polyadenylation sequence is also likely to halt transcription at the appropriate point, making it so that there is not much more to the mRNA in any case.

]]> By: David http://neurodudes.com/2006/07/02/a-genetically-encoded-fluorescent-amino-acid/comment-page-1/#comment-6339 David Fri, 21 Jul 2006 01:35:56 +0000 http://neurodudes.com/2006/07/02/a-genetically-encoded-fluorescent-amino-acid/#comment-6339 Yeah it's just too bad that amber (TAG) is a stop codon - that means that this amino acid would lead to unnatural read-through of stop codons in the whole lot of proteins that use amber for their termination sequence. The yeast are (apparently) robust enough in the scheme of things though - they didn't report any prion-like aggregates. On the other hand, we could engineer an organism that doesn't use amber for a stop codon (why is it that we need multiple stop codons anyway)? Yeah it’s just too bad that amber (TAG) is a stop codon – that means that this amino acid would lead to unnatural read-through of stop codons in the whole lot of proteins that use amber for their termination sequence. The yeast are (apparently) robust enough in the scheme of things though – they didn’t report any prion-like aggregates.

On the other hand, we could engineer an organism that doesn’t use amber for a stop codon (why is it that we need multiple stop codons anyway)?

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