Comments on: Cautionary note on FRET http://neurodudes.com/2007/02/21/cautionary-note-on-fret/ at the intersection of neuroscience and AI. Sat, 17 Jul 2010 08:17:33 +0000 http://wordpress.org/?v=2.9.2 hourly 1 By: Andrew Hires http://neurodudes.com/2007/02/21/cautionary-note-on-fret/comment-page-1/#comment-47050 Andrew Hires Tue, 06 Mar 2007 09:52:51 +0000 http://neurodudes.com/2007/02/21/cautionary-note-on-fret/#comment-47050 While I was at first quite skeptical of the reported results, my attitude has changed after trying to replicate their results. Applying ATP to purified GluSnFR gave very similar quenching effects. The authors of this study may be onto something significant. Or we all could be missing an obscure artifact. For a more complete breakdown of the paper and to see some new data supporting their results, check out my <a href="http://brainwindows.wordpress.com/2007/03/06/atp-and-fret-a-cautionary-note-confirmed/">weblog post</a>. While I was at first quite skeptical of the reported results, my attitude has changed after trying to replicate their results. Applying ATP to purified GluSnFR gave very similar quenching effects. The authors of this study may be onto something significant. Or we all could be missing an obscure artifact. For a more complete breakdown of the paper and to see some new data supporting their results, check out my weblog post.

]]> By: Brain Windows http://neurodudes.com/2007/02/21/cautionary-note-on-fret/comment-page-1/#comment-47044 Brain Windows Tue, 06 Mar 2007 09:38:50 +0000 http://neurodudes.com/2007/02/21/cautionary-note-on-fret/#comment-47044 <strong>ATP and FRET - A cautionary note, confirmed?</strong> In the February issue of Nature Biotechnology, a correspondence piece noted some very surprising findings regarding the sensitivity of genetically-encoded fluorescence resonance energy transfer (FRET) indicators to adenosine triphosphate. During the ... ATP and FRET – A cautionary note, confirmed?

In the February issue of Nature Biotechnology, a correspondence piece noted some very surprising findings regarding the sensitivity of genetically-encoded fluorescence resonance energy transfer (FRET) indicators to adenosine triphosphate.
During the …

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